Hotspot

atpB-rbcL

LSC · 56494-57275 · 782 bp

Back to Chionanthus

Pi 0.0144

MarkerSeek score 63.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 50
pi: 0.0144
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGGCCCAGGTTTTGTTT	GTAAGTCCATCGGTCCACAC	45.0	55.0	58.0	58.0	1048-1081 bp	1.0	88.3
atpB-rbcL_p2	ATACGGCCCAGGTTTTGTTT	GGTAGCATCGCCCTTTGTAA	45.0	50.0	58.0	58.0	1080-1113 bp	1.0	87.9
atpB-rbcL_p3	ATACGGCCCAGGTTTTGTTT	GGGGACGACCATACTTGTTC	45.0	55.0	58.0	58.0	1329-1362 bp	1.0	87.1
atpB-rbcL_p4	ATACGGCCCAGGTTTTGTTT	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	1479-1512 bp	1.0	86.6
atpB-rbcL_p5	ATACGGCCCAGGTTTTGTTT	GTACCCGCAGTAGCATTCAA	45.0	50.0	58.0	58.0	1561-1594 bp	1.0	86.5