Hotspot

atpH-atpI

LSC · 14705-15775 · 1071 bp

Back to Chionanthus

Pi 0.0123

MarkerSeek score 74.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 72
pi: 0.0123
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1200-1219 bp	1.0	82.8
atpH-atpI_p2	CAGCAGCAATAACAGAAGCG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1210-1229 bp	1.0	82.2
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	TCCCTGTCATGTTCCTTGGA	55.0	50.0	58.1	58.3	1285-1304 bp	1.0	82.1
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	TCCCTGTCATGTTCCTTGGA	55.0	50.0	57.9	58.3	1289-1308 bp	1.0	82.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1217-1236 bp	1.0	81.8