Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9438-10145 · 708 bp

Back to Chionanthus

Pi 0.015

MarkerSeek score 75.00

Primer available yes

Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name: trnS(GCU)-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 57
pi: 0.015
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(GCC)_p1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	797-825 bp	1.0	70.2
trnS(GCU)-trnG(GCC)_p2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	796-824 bp	1.0	70.2
trnS(GCU)-trnG(GCC)_p3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	57.9	56.6	861-889 bp	1.0	68.7
trnS(GCU)-trnG(GCC)_p4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	862-890 bp	1.0	67.9
trnS(GCU)-trnG(GCC)_p5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	863-891 bp	1.0	67.9