Hotspot

rbcL

LSC · 56926-58359 · 1434 bp

Back to Chimonocalamus

Pi 0.0022

MarkerSeek score 31.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 8
pi: 0.0022
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGTAGACCCTGTCGTTGTGA	TATGAAAATGCCGGGTGGAG	50.0	50.0	58.0	57.9	1982-1988 bp	1.0	80.4
rbcL_p2	GGGGACGAGGACTTGATTTC	TATGAAAATGCCGGGTGGAG	55.0	50.0	58.0	57.9	2569-2575 bp	1.0	80.1
rbcL_p3	AGTAGACCCTGTCGTTGTGA	GGCGATATACCACCCAAAGG	50.0	55.0	58.0	58.1	2003-2009 bp	1.0	79.9
rbcL_p4	GGGGACGAGGACTTGATTTC	GGCGATATACCACCCAAAGG	55.0	55.0	58.0	58.1	2590-2596 bp	1.0	79.7
rbcL_p5	TTTGTGGAGGATCTGTCCCT	TATGAAAATGCCGGGTGGAG	50.0	50.0	58.0	57.9	2458-2464 bp	0.9	75.9