Hotspot

rps18

LSC · 68167-68679 · 513 bp

Back to Chimonobambusa

Pi 0.0015

MarkerSeek score 35.50

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 513
variable_sites: 2
pi: 0.0015
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGAATCGCCACAATACACCC	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	1231-1273 bp	1.0	83.8
rps18_p2	CAAGCTAAGTCAGGCCTCAG	CGTTTGTGGATCACTCGGAT	55.0	50.0	58.0	58.0	1604-1646 bp	1.0	83.0
rps18_p3	CATAGAAAGAGGCTGGGCTG	CGTTTGTGGATCACTCGGAT	55.0	50.0	58.0	58.0	1575-1617 bp	1.0	82.9
rps18_p4	CAAGCTAAGTCAGGCCTCAG	GACGACGAACAAAAATGCGT	55.0	45.0	58.0	58.0	1744-1786 bp	1.0	82.7
rps18_p5	CATAGAAAGAGGCTGGGCTG	GACGACGAACAAAAATGCGT	55.0	45.0	58.0	58.0	1715-1757 bp	1.0	82.7