Hotspot

rpoC2

LSC · 27163-31716 · 4554 bp

Back to Chimonobambusa

Pi 0.000519

MarkerSeek score 49.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4554
variable_sites: 13
pi: 0.000519
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACCCTCCAAGTACTTCAAGG	TGTCCAAGGTGAATTATTGCC	50.0	42.9	56.5	56.5	161 bp	1.0	49.0
rpoC2_p2	AACCCTCCAAGTACTTCAAGG	TGTCCAAGGTGAATTATTGCC	47.6	42.9	57.2	56.5	162 bp	1.0	47.0
rpoC2_p3	ACCCTCCAAGTACTTCAAGG	TTGTCCAAGGTGAATTATTGCC	50.0	40.9	56.5	57.2	162 bp	1.0	46.7
rpoC2_p4	AACCCTCCAAGTACTTCAAGG	TTGTCCAAGGTGAATTATTGCC	47.6	40.9	57.2	57.2	163 bp	1.0	44.8
rpoC2_p5	AAACCCTCCAAGTACTTCAAGG	TGTCCAAGGTGAATTATTGCC	45.5	42.9	57.9	56.5	163 bp	1.0	44.6