Hotspot

rpoB

LSC · 21653-24880 · 3228 bp

Back to Chimonobambusa

Pi 0.000529

MarkerSeek score 50.70

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3228
variable_sites: 8
pi: 0.000529
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	AGAAGGATTTGGTGTTGCTCA	TTGTTGAGGGGAAACGAGTC	42.9	50.0	57.8	57.8	322 bp	1.0	68.7
rpoB_p2	AATGCTACGATCTGGGGAAA	TTGTTGAGGGGAAACGAGTC	45.0	50.0	56.6	57.8	240 bp	1.0	67.5
rpoB_p3	AGAAGGATTTGGTGTTGCTCA	GAGGGGAAACGAGTCCAATT	42.9	50.0	57.8	57.5	317 bp	1.0	66.7
rpoB_p4	AATGCTACGATCTGGGGAAA	GAGGGGAAACGAGTCCAATT	45.0	50.0	56.6	57.5	235 bp	1.0	65.6
rpoB_p5	AGAAGGATTTGGTGTTGCTCA	TGAGGGGAAACGAGTCCAAT	42.9	50.0	57.8	58.6	318 bp	1.0	65.5