Hotspot

rpl14

LSC · 79804-80175 · 372 bp

Back to Chimonobambusa

Pi 0.00293
MarkerSeek score 30.70
Primer available yes
Label rpl14

Feature Metadata

label_name
rpl14
feature_type
gene
region
LSC
length_bp
372
variable_sites
6
pi
0.00293
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14_p1 TCTTTGATCTATATCTCGCCT TCTTTCATCCTTTTGCATAGA 38.1 33.3 53.4 52.8 447 bp 1.0 41.1
rpl14_p2 TCTTTGATCTATATCTCGCCTACT TCTTTCATCCTTTTGCATAGA 37.5 33.3 56.4 52.8 447 bp 1.0 41.1
rpl14_p3 TCTTTGATCTATATCTCGCCT TCTTTCATCCTTTTGCATAGAA 38.1 31.8 53.4 53.7 447 bp 1.0 41.1
rpl14_p4 TCTTTGATCTATATCTCGCCT TTCTTTCATCCTTTTGCATAGA 38.1 31.8 53.4 53.7 448 bp 1.0 41.1
rpl14_p5 TTCTTTGATCTATATCTCGCCT TCTTTCATCCTTTTGCATAGA 36.4 33.3 54.2 52.8 448 bp 1.0 41.1