Hotspot

ndhF

SSC · 105112-107331 · 2220 bp

Back to Chimonobambusa

Pi 0.000246

MarkerSeek score 36.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 3
pi: 0.000246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAGAGATGGGGGAAGAAGA	TGGTTTGGTGAATCGGAAACA	55.0	42.9	57.8	58.3	2363 bp	1.0	67.9
ndhF_p2	GGAGAGATGGGGGAAGAAGA	GGTTTGGTGAATCGGAAACA	55.0	45.0	57.8	56.5	2362 bp	1.0	67.0
ndhF_p3	GGAGAGATGGGGGAAGAAGA	TGGTTTGGTGAATCGGAAAC	55.0	45.0	57.8	56.5	2363 bp	1.0	67.0
ndhF_p4	GGAGACGGGGGAATTCCTTA	TGGTTTGGTGAATCGGAAACA	55.0	42.9	58.5	58.3	2312 bp	1.0	65.4
ndhF_p5	AGAAAAGGGGGAGAGATGGG	TGGTTTGGTGAATCGGAAACA	55.0	42.9	58.4	58.3	2372 bp	0.909091	62.5