Hotspot

ndhH-ndhF

SSC · 105004-105111 · 108 bp

Back to Chimonobambusa

Pi 0.0236

MarkerSeek score 64.60

Primer available yes

Label ndhH-ndhF

Feature Metadata

label_name: ndhH-ndhF
feature_type: igs
region: SSC
length_bp: 108
variable_sites: 14
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ndhF_p1	AAAATCGCGGAAAACCGAAC	AAATATGTAGGGGGAGGGCG	45.0	55.0	58.0	58.9	228-233 bp	1.0	79.9
ndhH-ndhF_p2	TCGCGGAAAACCGAACTATT	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.9	58.9	224-229 bp	1.0	79.7
ndhH-ndhF_p3	AATCGCGGAAAACCGAACTA	AAATATGTAGGGGGAGGGCG	45.0	55.0	57.9	58.9	226-231 bp	1.0	79.6
ndhH-ndhF_p4	AAAATCGCGGAAAACCGAAC	GTAGGGGGAGGGCGAATAT	45.0	57.9	58.0	57.9	222-227 bp	1.0	78.9
ndhH-ndhF_p5	AAAATCGCGGAAAACCGAAC	GTAGGGGGAGGGCGAATATC	45.0	60.0	58.0	59.1	222-227 bp	1.0	78.8