Hotspot

rpl14-rpl16

LSC · 80176-80284 · 109 bp

Back to Chimonobambusa

Pi 0.00467

MarkerSeek score 51.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 2
pi: 0.00467
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCAGCTCCTATGACTCGAA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.2	58.2	245-248 bp	1.0	78.2
rpl14-rpl16_p2	CGAGCTCCACTGTTATCTGC	TAGCTGCCAGTAAAATGCCC	55.0	50.0	58.4	58.2	211-214 bp	1.0	76.9
rpl14-rpl16_p3	TAGCAGCTCCTATGACTCGA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.4	58.2	246-249 bp	1.0	74.9
rpl14-rpl16_p4	AGCAGCTCCTATGACTCGAA	TGAAATGGGCGGAGTATCTG	50.0	50.0	58.2	57.4	292-295 bp	1.0	74.8
rpl14-rpl16_p5	AGCAGCTCCTATGACTCGAA	GCCAGTAAAATGCCCATACG	50.0	50.0	58.2	57.2	240-243 bp	1.0	73.9