Hotspot

rbcL-psaI

LSC · 58367-59652 · 1286 bp

Back to Chimonobambusa

Pi 0.00308

MarkerSeek score 51.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 11
pi: 0.00308
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAATTTGAGTTCGAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.1	58.0	1394-1395 bp	1.0	79.6
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1424-1425 bp	1.0	79.1
rbcL-psaI_p3	AAATTTGAGTTCGAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.1	57.9	1400-1401 bp	1.0	79.0
rbcL-psaI_p4	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1430-1431 bp	1.0	78.5
rbcL-psaI_p5	CAAATTTGAGTTCGAGCCGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1395-1396 bp	1.0	78.5