Hotspot

trnG-UCC-trnT-GGU

LSC · 14351-16313 · 1963 bp

Back to Chimonobambusa

Pi 0.00229

MarkerSeek score 65.00

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1963
variable_sites: 14
pi: 0.00229
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	38.1	52.4	55.5	58.1	1475-2143 bp	1.0	47.1
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	38.1	47.6	55.5	57.8	1488-2156 bp	1.0	46.3
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	38.1	47.6	55.5	57.8	1489-2157 bp	1.0	46.3
trnG-UCC-trnT-GGU_p4	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	1478-2146 bp	1.0	46.0
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	1477-2145 bp	1.0	46.0