Hotspot

petA

LSC · 62861-63823 · 963 bp

Back to Chimonanthus

Pi 0.0027

MarkerSeek score 54.60

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 7
pi: 0.0027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	ACAATGGCAGAATCGTGGAT	GGCCCTTTTTACCAACCTGA	45.0	50.0	57.6	58.0	1079 bp	1.0	77.1
petA_p2	ACAATGGCAGAATCGTGGAT	CAACCTGATGAATCGGTCGA	45.0	50.0	57.6	57.7	1067 bp	1.0	74.8
petA_p3	ACAATGGCAGAATCGTGGAT	ACCAACCTGATGAATCGGTC	45.0	50.0	57.6	57.6	1069 bp	1.0	73.9
petA_p4	ATGGCAGAATCGTGGATAGG	GGCCCTTTTTACCAACCTGA	50.0	50.0	57.2	58.0	1076 bp	1.0	73.7
petA_p5	GCAGAATCGTGGATAGGGAA	GGCCCTTTTTACCAACCTGA	50.0	50.0	57.1	58.0	1073 bp	1.0	73.2