Hotspot

clpP

LSC · 71025-73032 · 2008 bp

Back to Chimonanthus

Pi 0.00209

MarkerSeek score 52.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2008
variable_sites: 10
pi: 0.00209
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TTTAGATCGATCCTAACCGG	ACGTTTCCACATCAAAGTGA	45.0	40.0	54.1	55.5	2177-2179 bp	1.0	40.6
clpP_p2	TTTAGATCGATCCTAACCGG	ACGTTTCCACATCAAAGTGAAG	45.0	40.9	54.1	57.4	2177-2179 bp	1.0	40.6
clpP_p3	TTTAGATCGATCCTAACCGG	CGTTTCCACATCAAAGTGAAGT	45.0	40.9	54.1	57.4	2176-2178 bp	1.0	40.6
clpP_p4	TTTAGATCGATCCTAACCGG	ACGTTTCCACATCAAAGTGAA	45.0	38.1	54.1	56.3	2177-2179 bp	1.0	40.6
clpP_p5	TTTAGATCGATCCTAACCGG	ACGGCAATATTCTGAAGACA	45.0	40.0	54.1	54.8	2216-2218 bp	1.0	40.6