Hotspot

trnN-GUU-trnR-ACG

IRa · 130413-131015 · 603 bp

Back to Chimonanthus

Pi 0.0253

MarkerSeek score 74.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 603
variable_sites: 30
pi: 0.0253
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	744 bp	1.0	85.0
trnN-GUU-trnR-ACG_p2	CGGTTGGCTGTTAACTGACT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	714 bp	1.0	84.4
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	695 bp	1.0	84.3
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	708 bp	1.0	84.2
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	781 bp	1.0	84.1