Hotspot

trnR-ACG-trnN-GUU

IRb · 108672-109274 · 603 bp

Back to Chimonanthus

Pi 0.0253

MarkerSeek score 74.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 603
variable_sites: 30
pi: 0.0253
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	744 bp	1.0	85.0
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	55.0	50.0	57.8	58.1	714 bp	1.0	84.4
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	695 bp	1.0	84.3
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	708 bp	1.0	84.2
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	781 bp	1.0	84.1