Hotspot

accD-psaI

LSC · 59703-60221 · 519 bp

Back to Chimonanthus

Pi 0.00501

MarkerSeek score 54.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 6
pi: 0.00501
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAGAGCGCTAGGTTCGGTTA	GCCATTGCAATTGCTGGAAA	50.0	45.0	58.0	58.2	627-718 bp	1.0	78.8
accD-psaI_p2	CAAGTAGAGCGCTAGGTTCG	GCCATTGCAATTGCTGGAAA	55.0	45.0	58.2	58.2	631-722 bp	1.0	77.8
accD-psaI_p3	GCTACACGGTTTCTTTCCCT	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	663-754 bp	1.0	77.7
accD-psaI_p4	TCGGCTACACGGTTTCTTTC	GCCATTGCAATTGCTGGAAA	50.0	45.0	58.2	58.2	666-757 bp	1.0	77.4
accD-psaI_p5	GAGCGCTAGGTTCGGTTATT	GCCATTGCAATTGCTGGAAA	50.0	45.0	57.8	58.2	625-716 bp	1.0	77.4