Hotspot

rpoB-trnC-GCA

LSC · 27254-28417 · 1164 bp

Back to Chimonanthus

Pi 0.00207

MarkerSeek score 52.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 6
pi: 0.00207
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TGAAGTTCTTCCGTCAAGCC	AGCAGGGTAAGAGATTCCGA	50.0	50.0	58.1	57.8	1352-1363 bp	1.0	78.5
rpoB-trnC-GCA_p2	TGAAGTTCTTCCGTCAAGCC	TTGGCGAGTTCTGTTAGCAG	50.0	50.0	58.1	58.2	1367-1378 bp	1.0	78.2
rpoB-trnC-GCA_p3	TGAAGTTCTTCCGTCAAGCC	CTGGGGAGAAAGGATTTGCA	50.0	50.0	58.1	57.8	1298-1309 bp	1.0	78.1
rpoB-trnC-GCA_p4	TGAAGTTCTTCCGTCAAGCC	TGGGGAGAAAGGATTTGCAG	50.0	50.0	58.1	57.8	1297-1308 bp	1.0	78.1
rpoB-trnC-GCA_p5	TGAAGTTCTTCCGTCAAGCC	AGAAAGGATTTGCAGTCCCC	50.0	50.0	58.1	57.8	1292-1303 bp	1.0	78.1