Hotspot

rps16-trnQ-UUG

LSC · 6114-7250 · 1137 bp

Back to Chimonanthus

Pi 0.00457
MarkerSeek score 57.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1137
variable_sites
13
pi
0.00457
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1196-1229 bp 1.0 80.2
rps16-trnQ-UUG_p2 TGCAAGAATCCACAACCGAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1226-1259 bp 1.0 79.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1200-1233 bp 1.0 79.2
rps16-trnQ-UUG_p4 TGCAAGAATCCACAACCGAT CTCGGAGGTTCGAATCCTTC 45.0 55.0 57.8 57.8 1230-1263 bp 1.0 78.6
rps16-trnQ-UUG_p5 AATGGTGGGTGCAAGAATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1235-1268 bp 1.0 77.8