Hotspot

rpl16

LSC · 80028-81654 · 1627 bp

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Pi 0.0142

MarkerSeek score 58.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1627
variable_sites: 53
pi: 0.0142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGGGCACTGCTTCTTTGATT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2228-2265 bp	1.0	83.4
rpl16_p2	TTGGGCACTGCTTCTTTGAT	GCAAATTGCAGGACGTATCG	45.0	50.0	58.0	57.9	2229-2266 bp	1.0	83.4
rpl16_p3	TGGGCACTGCTTCTTTGATT	ACGGAAAAGAGATTGCACGT	45.0	45.0	58.0	58.1	2208-2245 bp	1.0	83.2
rpl16_p4	TTGGGCACTGCTTCTTTGAT	ACGGAAAAGAGATTGCACGT	45.0	45.0	58.0	58.1	2209-2246 bp	1.0	83.2
rpl16_p5	TGGGCACTGCTTCTTTGATT	AAGAGATTGCACGTGTCGAA	45.0	45.0	58.0	57.9	2202-2239 bp	1.0	83.0