Hotspot

rps14-psaB

LSC · 37923-38061 · 139 bp

Back to Chiloschista

Pi 0.0158

MarkerSeek score 70.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 5
pi: 0.0158
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	GTTCCAATTTCTGGCGCTTC	TTGATTGCCTCCACATCAGG	50.0	50.0	58.0	57.9	217-224 bp	1.0	83.1
rps14-psaB_p2	GTTCCAATTTCTGGCGCTTC	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.0	58.1	301-308 bp	1.0	82.3
rps14-psaB_p3	TCCAATTTCTGGCGCTTCTT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	58.1	58.1	299-306 bp	1.0	81.8
rps14-psaB_p4	TTCCAATTTCTGGCGCTTCT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	58.1	58.1	300-307 bp	1.0	81.7
rps14-psaB_p5	CTCAACGAGGGAACTTTGCT	CCAGTGGCTCTTTCCATTGT	50.0	50.0	58.1	58.1	362-369 bp	1.0	81.0