Hotspot

atpF-atpH

LSC · 13799-13963 · 165 bp

Back to Chiloschista

Pi 0.0206

MarkerSeek score 64.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 7
pi: 0.0206
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAACTCGAAACTACCAGCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.2	58.0	282-305 bp	1.0	80.8
atpF-atpH_p2	ACGGGAACAAAAGAATCGGT	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.7	58.0	248-271 bp	1.0	80.7
atpF-atpH_p3	AAACTCGAAACTACCAGCGG	GCGGAGGGTAAAATACGAGG	50.0	55.0	58.2	58.1	342-365 bp	1.0	79.5
atpF-atpH_p4	ACGGGAACAAAAGAATCGGT	GCGGAGGGTAAAATACGAGG	45.0	55.0	57.7	58.1	308-331 bp	1.0	79.3
atpF-atpH_p5	AAACTCGAAACTACCAGCGG	GGTCGTGGCATTAGCTCTTT	50.0	50.0	58.2	58.3	271-294 bp	1.0	79.1