Hotspot

psbK-psbI

LSC · 7517-8027 · 511 bp

Back to Chiloschista

Pi 0.022

MarkerSeek score 68.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 24
pi: 0.022
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCGTTAGATAAG	50.0	55.0	57.9	57.6	569-630 bp	1.0	83.8
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	589-650 bp	1.0	83.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	566-627 bp	1.0	81.7
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	567-628 bp	1.0	81.7
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GCCCCGGATCGTTAGATAAG	45.0	55.0	58.3	57.6	568-629 bp	1.0	81.7