Hotspot

rps16-trnQ-UUG

LSC · 6002-6878 · 877 bp

Back to Chiloschista

Pi 0.0144

MarkerSeek score 63.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 30
pi: 0.0144
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	962-973 bp	1.0	83.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	939-950 bp	1.0	82.8
rps16-trnQ-UUG_p3	CCACAGCCAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	960-971 bp	1.0	82.5
rps16-trnQ-UUG_p4	ATCCACAGCCAATCATGTCC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.9	57.8	966-977 bp	1.0	82.2
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	950-961 bp	1.0	82.2