Hotspot

rpl14

LSC · 78918-79289 · 372 bp

Back to Chikusichloa

Pi 0

MarkerSeek score 22.40

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 372
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	TCTTTGATCTATATCTTGTCTACT	AAACCAGGCTCTTCTTTCTG	29.2	45.0	52.3	55.6	480 bp	1.0	40.0
rpl14_p2	TCTTTGATCTATATCTTGTCTACT	CAGGCTCTTCTTTCTGGAAAG	29.2	47.6	52.3	56.5	476 bp	1.0	40.0
rpl14_p3	TCTTTGATCTATATCTTGTCTACT	AAAACCAGGCTCTTCTTTCTG	29.2	42.9	52.3	56.4	481 bp	1.0	40.0
rpl14_p4	TCTTTGATCTATATCTTGTCTACTAGT	AAACCAGGCTCTTCTTTCTG	29.6	45.0	55.0	55.6	480 bp	1.0	40.0
rpl14_p5	TCTTTGATCTATATCTTGTCTACTAGT	CAGGCTCTTCTTTCTGGAAAG	29.6	47.6	55.0	56.5	476 bp	1.0	40.0