Hotspot

rpl14-rpl16

LSC · 79290-79411 · 122 bp

Back to Chikusichloa

Pi 0.0165
MarkerSeek score 62.80
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
122
variable_sites
2
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 AGCAGCTCCTATGACTCGAA TAGCTGCCAGTAAAATGCCC 50.0 50.0 58.2 58.2 258-259 bp 1.0 77.2
rpl14-rpl16_p2 AACGTCACCAATACGAGCAT TAGCTGCCAGTAAAATGCCC 45.0 50.0 57.6 58.2 291-292 bp 1.0 75.9
rpl14-rpl16_p3 CGAGCTCCACTGTTATCTGC TAGCTGCCAGTAAAATGCCC 55.0 50.0 58.4 58.2 224-225 bp 1.0 75.7
rpl14-rpl16_p4 TAGCAGCTCCTATGACTCGA TAGCTGCCAGTAAAATGCCC 50.0 50.0 57.4 58.2 259-260 bp 1.0 73.9
rpl14-rpl16_p5 AGCAGCTCCTATGACTCGAA GCCAGTAAAATGCCCATACG 50.0 50.0 58.2 57.2 253-254 bp 1.0 72.9