Hotspot

rpl36-infA

LSC · 77844-77951 · 108 bp

Back to Chikusichloa

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 2
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TGACTCTAATTCGTCCACGC	TCGAAACGTACCGAAGACTC	50.0	50.0	57.7	57.4	250 bp	1.0	73.6
rpl36-infA_p2	TGACTCTAATTCGTCCACGC	CGAAACGTACCGAAGACTCG	50.0	55.0	57.7	58.8	249 bp	1.0	72.4
rpl36-infA_p3	GACTCTAATTCGTCCACGCC	TCGAAACGTACCGAAGACTC	55.0	50.0	58.4	57.4	249 bp	1.0	72.3
rpl36-infA_p4	GACTCTAATTCGTCCACGCC	CGAAACGTACCGAAGACTCG	55.0	55.0	58.4	58.8	248 bp	1.0	71.1
rpl36-infA_p5	TGACTCTAATTCGTCCACGC	AGGATTCGAAACGTACCGAA	50.0	45.0	57.7	57.0	255 bp	1.0	70.3