Hotspot

trnT-GGU-trnE-UUC

LSC · 15383-15909 · 527 bp

Back to Chikusichloa

Pi 0

MarkerSeek score 38.50

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	CTCAGCGGTAGAGTAATGCC	GCTGCCTCCTTGAAAGAGAG	55.0	55.0	57.9	58.3	637 bp	1.0	76.8
trnT-GGU-trnE-UUC_p2	CTCAGCGGTAGAGTAATGCC	TGTCCTGAACCACTAGACGA	55.0	50.0	57.9	57.7	616 bp	1.0	76.6
trnT-GGU-trnE-UUC_p3	AGCCTTTTTAACTCAGCGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	57.7	58.3	648 bp	1.0	75.7
trnT-GGU-trnE-UUC_p4	AGCCTTTTTAACTCAGCGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	57.7	57.7	627 bp	1.0	75.6
trnT-GGU-trnE-UUC_p5	CTCAGCGGTAGAGTAATGCC	AACCACTAGACGATAGGGGC	55.0	55.0	57.9	58.6	609 bp	1.0	74.2