Hotspot

rps16-trnQ-UUG

LSC · 5580-6663 · 1084 bp

Back to Chikusichloa

Pi 0.00277

MarkerSeek score 60.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 3
pi: 0.00277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAATCGATCGTGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1179 bp	1.0	79.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1158 bp	1.0	78.5
rps16-trnQ-UUG_p3	CCACAATCGATCGTGTCCTT	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.0	57.8	1183 bp	1.0	78.4
rps16-trnQ-UUG_p4	ATCCACAATCGATCGTGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1181 bp	1.0	77.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1162 bp	1.0	77.4