Hotspot

trnK-UUU-rps16

LSC · 3940-4502 · 563 bp

Back to Chikusichloa

Pi 0.00355
MarkerSeek score 60.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
563
variable_sites
2
pi
0.00355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ATCAGTCGCGGTCTTCTAGA GGTGCTCAACCTACAAGAACT 50.0 47.6 58.0 57.9 764 bp 1.0 70.9
trnK-UUU-rps16_p2 ATCAGTCGCGGTCTTCTAGA GGTGCTCAACCTACAAGAAC 50.0 50.0 58.0 56.4 764 bp 1.0 66.8
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAAGAACT 50.0 47.6 58.6 57.9 689 bp 1.0 66.6
trnK-UUU-rps16_p4 AGTCGCGGTCTTCTAGACTC GGTGCTCAACCTACAAGAACT 55.0 47.6 58.6 57.9 761 bp 1.0 66.2
trnK-UUU-rps16_p5 GTCGCGGTCTTCTAGACTCT GGTGCTCAACCTACAAGAACT 55.0 47.6 58.6 57.9 760 bp 1.0 66.2