Hotspot

syfB

Genome · 40038-42050 · 2013 bp

Back to Chiharaea

Pi 0.00944

MarkerSeek score 59.80

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2013
variable_sites: 19
pi: 0.00944
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	ACAAAAGCCTCTTCTTAGTT	AACAGAACCCGGCTTATGTC	35.0	50.0	52.3	57.9	2113 bp	1.0	40.7
syfB_p2	ACAAAAGCCTCTTCTTAGTT	GAACAGAACCCGGCTTATGT	35.0	50.0	52.3	57.9	2114 bp	1.0	40.7
syfB_p3	TGGAACGTTATTCAAAAGAGA	AACAGAACCCGGCTTATGTC	33.3	50.0	53.1	57.9	2148 bp	1.0	40.7
syfB_p4	TGGAACGTTATTCAAAAGAGA	GAACAGAACCCGGCTTATGT	33.3	50.0	53.1	57.9	2149 bp	1.0	40.7
syfB_p5	ACAAAAGCCTCTTCTTAGTT	CAGAACCCGGCTTATGTCTT	35.0	50.0	52.3	57.6	2111 bp	1.0	40.7