Hotspot

sufB

Genome · 132040-133503 · 1464 bp

Back to Chiharaea

Pi 0.0102

MarkerSeek score 50.20

Primer available yes

Label sufB

Feature Metadata

label_name: sufB
feature_type: gene
region: Genome
length_bp: 1464
variable_sites: 15
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
sufB_p1	CGCCAATGTACTTTTGCCTG	CAACCATTTAGCTGGCTTGC	50.0	50.0	58.0	58.0	2191 bp	1.0	80.7
sufB_p2	ATGACTAGCGCGTTCTTCTG	CAACCATTTAGCTGGCTTGC	50.0	50.0	58.1	58.0	2293 bp	1.0	80.2
sufB_p3	CATGACTAGCGCGTTCTTCT	CAACCATTTAGCTGGCTTGC	50.0	50.0	58.1	58.0	2294 bp	1.0	80.2
sufB_p4	CGCCAATGTACTTTTGCCTG	AACCATTTAGCTGGCTTGCT	50.0	45.0	58.0	58.1	2190 bp	1.0	80.1
sufB_p5	CGCCAATGTACTTTTGCCTG	ACCATTTAGCTGGCTTGCTT	50.0	45.0	58.0	58.1	2189 bp	1.0	80.1