Hotspot

rps14

Genome · 83780-84082 · 303 bp

Back to Chiharaea

Pi 0.0099

MarkerSeek score 45.00

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 3
pi: 0.0099
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	GCAGTATCGTCGGGGTAATC	GGCGAGGATATTGGCAAGAA	55.0	50.0	57.9	58.0	745 bp	1.0	80.2
rps14_p2	GCAGTATCGTCGGGGTAATC	AAGGTGCTTTGGATGCAAGA	55.0	45.0	57.9	58.0	1138 bp	1.0	80.1
rps14_p3	GAAGCTAGCGTAACAGGCAT	AAGGTGCTTTGGATGCAAGA	50.0	45.0	58.1	58.0	1734 bp	1.0	80.0
rps14_p4	GAAGCTAGCGTAACAGGCAT	GGCGAGGATATTGGCAAGAA	50.0	50.0	58.1	58.0	1341 bp	1.0	79.9
rps14_p5	GCAAAGTTACCGGAAGCCTA	AAGGTGCTTTGGATGCAAGA	50.0	45.0	57.9	58.0	1392 bp	1.0	79.7