Hotspot

orf702

Genome · 136632-138740 · 2109 bp

Back to Chiharaea

Pi 0.0133

MarkerSeek score 51.70

Primer available yes

Label orf702

Feature Metadata

label_name: orf702
feature_type: gene
region: Genome
length_bp: 2109
variable_sites: 28
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf702_p1	TCAGATGAAAAGCAAATGCCA	AGTGCTTCAATCTCCGCATT	38.1	45.0	56.7	57.9	2854 bp	0.5	40.6
orf702_p2	TCAGATGAAAAGCAAATGCCA	GCTTCAATCTCCGCATTTGG	38.1	50.0	56.7	57.8	2851 bp	0.5	39.9
orf702_p3	TCAGATGAAAAGCAAATGCCA	CTAGTGCTTCAATCTCCGCA	38.1	50.0	56.7	57.7	2856 bp	0.5	39.1
orf702_p4	TCAGATGAAAAGCAAATGCCAA	AGTGCTTCAATCTCCGCATT	36.4	45.0	57.4	57.9	2854 bp	0.5	38.2
orf702_p5	TTCAGATGAAAAGCAAATGCCA	AGTGCTTCAATCTCCGCATT	36.4	45.0	57.4	57.9	2855 bp	0.5	38.2