Hotspot

orf395

Genome · 140338-141525 · 1188 bp

Back to Chiharaea

Pi 0.0118

MarkerSeek score 62.00

Primer available yes

Label orf395

Feature Metadata

label_name: orf395
feature_type: gene
region: Genome
length_bp: 1188
variable_sites: 14
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf395_p1	AGTTTAGCAAACATACTATGCCT	CGTTCTTTTCAGAAAGGCGG	34.8	50.0	56.0	57.7	1354 bp	1.0	40.8
orf395_p2	AGTTTAGCAAACATACTATGCCT	AACCAGCGACGTTCTTTTCA	34.8	45.0	56.0	58.3	1363 bp	1.0	40.8
orf395_p3	AGTTTAGCAAACATACTATGCCT	CCAGCGACGTTCTTTTCAGA	34.8	50.0	56.0	58.5	1361 bp	1.0	40.8
orf395_p4	AAGTTTAGCAAACATACTATGCCT	CGTTCTTTTCAGAAAGGCGG	33.3	50.0	56.7	57.7	1355 bp	1.0	40.8
orf395_p5	AAGTTTAGCAAACATACTATGCCT	AACCAGCGACGTTCTTTTCA	33.3	45.0	56.7	58.3	1364 bp	1.0	40.8