Hotspot

rps4-orf448

Genome · 149585-149624 · 40 bp

Back to Chiharaea

Pi 0.05

MarkerSeek score 72.10

Primer available yes

Label rps4-orf448

Feature Metadata

label_name: rps4-orf448
feature_type: igs
region: Genome
length_bp: 40
variable_sites: 2
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-orf448_p1	CGGGATTATTGAAAGAGAATGGG	TCAACAGCAACAAATTCAACCT	43.5	36.4	57.3	57.3	234 bp	1.0	40.7
rps4-orf448_p2	CGGGATTATTGAAAGAGAATGGG	TCAACAGCAACAAATTCAACC	43.5	38.1	57.3	55.8	234 bp	1.0	40.7
rps4-orf448_p3	CGGGATTATTGAAAGAGAATGGG	CAACAGCAACAAATTCAACCT	43.5	38.1	57.3	55.8	233 bp	1.0	40.7
rps4-orf448_p4	CGGGATTATTGAAAGAGAATGGG	ATCAACAGCAACAAATTCAACCT	43.5	34.8	57.3	57.7	235 bp	1.0	40.7
rps4-orf448_p5	ACGGGATTATTGAAAGAGAATGG	TCAACAGCAACAAATTCAACCT	39.1	36.4	56.6	57.3	235 bp	1.0	40.7