Hotspot

atpE-atpB

Genome · 110576-110587 · 12 bp

Back to Chiharaea

Pi 0.0833

MarkerSeek score 71.50

Primer available yes

Label atpE-atpB

Feature Metadata

label_name: atpE-atpB
feature_type: igs
region: Genome
length_bp: 12
variable_sites: 1
pi: 0.0833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-atpB_p1	TGCTGGGCAAAATGACTTCT	ACACTATCGTACGGCACAAC	45.0	50.0	58.0	58.0	419 bp	1.0	80.3
atpE-atpB_p2	TGCTGGGCAAAATGACTTCT	CACTATCGTACGGCACAACA	45.0	50.0	58.0	58.0	418 bp	1.0	80.3
atpE-atpB_p3	GGCAAAATGACTTCTTCGGC	ACACTATCGTACGGCACAAC	50.0	50.0	58.0	58.0	414 bp	1.0	80.1
atpE-atpB_p4	CATGCTTGCTGGAGATCCAT	ACACTATCGTACGGCACAAC	50.0	50.0	58.0	58.0	1014 bp	1.0	80.0
atpE-atpB_p5	CATGCTTGCTGGAGATCCAT	CACTATCGTACGGCACAACA	50.0	50.0	58.0	58.0	1013 bp	1.0	80.0