Hotspot

rpl35-rpl20

Genome · 100456-100486 · 31 bp

Back to Chiharaea

Pi 0.0323

MarkerSeek score 71.00

Primer available yes

Label rpl35-rpl20

Feature Metadata

label_name: rpl35-rpl20
feature_type: igs
region: Genome
length_bp: 31
variable_sites: 1
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl35-rpl20_p1	AAAGCCTGTCGAAGTCATTT	ACCTGTTGCTTAGCTACTCG	40.0	50.0	55.6	57.3	264 bp	1.0	55.6
rpl35-rpl20_p2	CATAAAGCCTGTCGAAGTCA	ACCTGTTGCTTAGCTACTCG	45.0	50.0	55.5	57.3	267 bp	1.0	55.0
rpl35-rpl20_p3	AAAGCCTGTCGAAGTCATTT	CCTGTTGCTTAGCTACTCGA	40.0	50.0	55.6	57.1	263 bp	1.0	53.4
rpl35-rpl20_p4	CATAAAGCCTGTCGAAGTCA	CCTGTTGCTTAGCTACTCGA	45.0	50.0	55.5	57.1	266 bp	1.0	52.7
rpl35-rpl20_p5	AAAGCCTGTCGAAGTCATTT	AACCTGTTGCTTAGCTACTCG	40.0	47.6	55.6	58.0	265 bp	1.0	52.6