Hotspot

acsF-petN

Genome · 97155-97596 · 442 bp

Back to Chiharaea

Pi 0.0113

MarkerSeek score 56.30

Primer available yes

Label acsF-petN

Feature Metadata

label_name: acsF-petN
feature_type: igs
region: Genome
length_bp: 442
variable_sites: 5
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acsF-petN_p1	TCGTTACTTGGTTTGATTGTGA	AAAAGAGCCCCACCCTAAAC	36.4	50.0	56.2	57.7	518 bp	1.0	48.2
acsF-petN_p2	TCGTTACTTGGTTTGATTGTGA	AAGAGCCCCACCCTAAACTA	36.4	50.0	56.2	57.6	516 bp	1.0	47.8
acsF-petN_p3	TCGTTACTTGGTTTGATTGTGA	AGAGCCCCACCCTAAACTAA	36.4	50.0	56.2	57.6	515 bp	1.0	47.8
acsF-petN_p4	TCGTTACTTGGTTTGATTGTGA	AAAGAGCCCCACCCTAAACT	36.4	50.0	56.2	58.5	517 bp	1.0	46.3
acsF-petN_p5	CGTTACTTGGTTTGATTGTGATGA	AAAAGAGCCCCACCCTAAAC	37.5	50.0	58.0	57.7	517 bp	1.0	46.3