Hotspot

rps19-rpl2

Genome · 78299-78306 · 8 bp

Back to Chiharaea

Pi 0.125

MarkerSeek score 74.50

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 8
variable_sites: 1
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCCGCTATTAGCAAACGCTT	CCAATGACACCTTGGGGAAA	45.0	50.0	58.0	58.0	630 bp	1.0	80.4
rps19-rpl2_p2	TCCGCTATTAGCAAACGCTT	ATGACACCTTGGGGAAAACC	45.0	50.0	58.0	58.0	627 bp	1.0	80.4
rps19-rpl2_p3	AAAGCAGGTCCGCTATTAGC	CCAATGACACCTTGGGGAAA	50.0	50.0	58.0	58.0	638 bp	1.0	80.2
rps19-rpl2_p4	AAAGCAGGTCCGCTATTAGC	ATGACACCTTGGGGAAAACC	50.0	50.0	58.0	58.0	635 bp	1.0	80.2
rps19-rpl2_p5	TCCGCTATTAGCAAACGCTT	TCACTTCTGGACCTAGTGCA	45.0	50.0	58.0	58.0	1019 bp	0.5	59.8