Hotspot

rpl6-rps8

Genome · 74395-74417 · 23 bp

Back to Chiharaea

Pi 0.0435

MarkerSeek score 66.80

Primer available yes

Label rpl6-rps8

Feature Metadata

label_name: rpl6-rps8
feature_type: igs
region: Genome
length_bp: 23
variable_sites: 1
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl6-rps8_p1	TCGAGTCTACAGGTATAGGACT	GCTCGATATTCCGGCTTAGG	45.5	55.0	56.7	58.0	111 bp	1.0	54.2
rpl6-rps8_p2	TCGAGTCTACAGGTATAGGACT	GGTGGCGAAGTATTGTGCTA	45.5	50.0	56.7	58.0	93 bp	1.0	54.2
rpl6-rps8_p3	TCGAGTCTACAGGTATAGGACT	TAGGTGGCGAAGTATTGTGC	45.5	50.0	56.7	58.0	95 bp	1.0	54.2
rpl6-rps8_p4	TCGAGTCTACAGGTATAGGACT	CGATATTCCGGCTTAGGTGG	45.5	55.0	56.7	57.9	108 bp	1.0	53.6
rpl6-rps8_p5	TCGAGTCTACAGGTATAGGACT	GATATTCCGGCTTAGGTGGC	45.5	55.0	56.7	58.2	107 bp	1.0	52.7