Hotspot

trnF(gaa)-clpC

Genome · 51983-52054 · 72 bp

Back to Chiharaea

Pi 0.0556
MarkerSeek score 69.90
Primer available yes
Label trnF(gaa)-clpC

Feature Metadata

label_name
trnF(gaa)-clpC
feature_type
igs
region
Genome
length_bp
72
variable_sites
4
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF(gaa)-clpC_p1 TGGTAGAGCAGTGGACTGAA AGGCGAAAAATTGGAATTACT 50.0 33.3 58.0 54.0 160 bp 1.0 42.3
trnF(gaa)-clpC_p2 TTGGTAGAGCAGTGGACTGA AGGCGAAAAATTGGAATTACT 50.0 33.3 58.0 54.0 161 bp 1.0 42.3
trnF(gaa)-clpC_p3 TCAGTTGGTAGAGCAGTGGA AGGCGAAAAATTGGAATTACT 50.0 33.3 58.0 54.0 165 bp 1.0 42.2
trnF(gaa)-clpC_p4 AGTTGGTAGAGCAGTGGACT AGGCGAAAAATTGGAATTACT 50.0 33.3 58.0 54.0 163 bp 1.0 42.2
trnF(gaa)-clpC_p5 ATCATAAGGCCGGGATAGCT AGGCGAAAAATTGGAATTACT 50.0 33.3 58.0 54.0 184 bp 1.0 41.8