Hotspot

trnN-GUU-trnR-ACG

Genome · 94562-95171 · 610 bp

Back to Chesneya

Pi 0.0612

MarkerSeek score 82.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: Genome
length_bp: 610
variable_sites: 36
pi: 0.0612
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	689-703 bp	1.0	83.7
trnN-GUU-trnR-ACG_p2	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	693-707 bp	1.0	82.8
trnN-GUU-trnR-ACG_p3	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.7	57.8	681-695 bp	1.0	82.4
trnN-GUU-trnR-ACG_p4	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	732-746 bp	1.0	82.1
trnN-GUU-trnR-ACG_p5	TTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.3	57.8	690-704 bp	1.0	79.6