Hotspot

trnL-UAA-trnN-GUU

Genome · 93809-94488 · 680 bp

Back to Chesneya

Pi 0.0675

MarkerSeek score 85.20

Primer available yes

Label trnL-UAA-trnN-GUU

Feature Metadata

label_name: trnL-UAA-trnN-GUU
feature_type: igs
region: Genome
length_bp: 680
variable_sites: 44
pi: 0.0675
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnN-GUU_p1	GTCTCGCGTGTCTACCATTT	AGGATTCGAACCTACGACCA	50.0	50.0	58.0	58.2	774-919 bp	1.0	85.5
trnL-UAA-trnN-GUU_p2	CTCGCGTGTCTACCATTTCA	AGGATTCGAACCTACGACCA	50.0	50.0	58.0	58.2	772-917 bp	1.0	85.5
trnL-UAA-trnN-GUU_p3	TACCATTTCACCACCAAGGC	AGGATTCGAACCTACGACCA	50.0	50.0	58.1	58.2	762-907 bp	1.0	85.0
trnL-UAA-trnN-GUU_p4	GTCTCGCGTGTCTACCATTT	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.0	57.7	782-927 bp	1.0	84.1
trnL-UAA-trnN-GUU_p5	CTCGCGTGTCTACCATTTCA	CCCCAAGTAGGATTCGAACC	50.0	55.0	58.0	57.7	780-925 bp	1.0	84.1