Hotspot

rps8-rpl14

Genome · 80043-80824 · 782 bp

Back to Chesneya

Pi 0.12
MarkerSeek score 84.00
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
Genome
length_bp
782
variable_sites
78
pi
0.12
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TCAGCATTGCGTATCAAGGT AGTTTCATTAGCACCTGAGGT 45.0 42.9 57.6 56.9 775-860 bp 1.0 71.8
rps8-rpl14_p2 GTCAGCATTGCGTATCAAGG AGTTTCATTAGCACCTGAGGT 50.0 42.9 57.3 56.9 776-861 bp 1.0 69.0
rps8-rpl14_p3 TCAGCATTGCGTATCAAGGT GTTTCATTAGCACCTGAGGT 45.0 45.0 57.6 55.4 774-859 bp 1.0 67.2
rps8-rpl14_p4 TCATGTCAGCATTGCGTATCA AGTTTCATTAGCACCTGAGGT 42.9 42.9 58.1 56.9 780-865 bp 1.0 66.2
rps8-rpl14_p5 CAGCATTGCGTATCAAGGTT AGTTTCATTAGCACCTGAGGT 45.0 42.9 56.8 56.9 774-859 bp 1.0 65.3