Hotspot

trnS-UGA-psbC

Genome · 26882-27090 · 209 bp

Back to Chesneya

Pi 0.0813

MarkerSeek score 80.20

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 209
variable_sites: 17
pi: 0.0813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.0	58.4	408-411 bp	1.0	79.8
trnS-UGA-psbC_p2	GCTGAGTGGTTGATAGCTCC	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	400-403 bp	1.0	79.6
trnS-UGA-psbC_p3	GGCTGAGTGGTTGATAGCTC	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	401-404 bp	1.0	79.6
trnS-UGA-psbC_p4	GGTTGATAGCTCCGGTCTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	393-396 bp	1.0	79.6
trnS-UGA-psbC_p5	GCTTTTGGAGAGATGGCTGA	TAGGTCATTTATGGCACGCG	50.0	50.0	57.9	58.4	415-418 bp	1.0	78.9