primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	ATCAGAGCACAGGGAGTCAT	GCCTAGGACTAGATCGTGGT	20	20	50.0	55.0	58.1	58.0	0.0	14.8	0.139086	10491	10925	1253-1255	1.000000	83.4
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	ATCAGAGCACAGGGAGTCAT	TGCCTAGGACTAGATCGTGG	20	20	50.0	55.0	58.1	58.0	0.0	14.8	0.140491	10491	10925	1254-1256	1.000000	83.3
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	ATCAGAGCACAGGGAGTCAT	TTTCATAGTTACCGTGCCCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136088	10491	10925	1294-1296	1.000000	83.2
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	GCACAAGGGCTATAGCTCAG	TTTCATAGTTACCGTGCCCG	20	20	55.0	50.0	58.1	58.0	26.2	0.0	0.136615	10491	10925	1745-1761	1.000000	83.1
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	GCACAAGGGCTATAGCTCAG	GCCTAGGACTAGATCGTGGT	20	20	55.0	55.0	58.1	58.0	26.2	14.8	0.139612	10491	10925	1704-1720	1.000000	83.1
trnL-CAA-trnT-UGU_p1	trnL-CAA-trnT-UGU	1	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	14100	15740	1575-1700	1.000000	87.5
trnL-CAA-trnT-UGU_p2	trnL-CAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	14100	15740	1607-1732	1.000000	86.6
trnL-CAA-trnT-UGU_p3	trnL-CAA-trnT-UGU	3	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	14100	15740	1581-1706	1.000000	86.1
trnL-CAA-trnT-UGU_p4	trnL-CAA-trnT-UGU	4	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	57.8	57.9	2.3	0.0	0.369229	14100	15740	1660-1785	1.000000	85.9
trnL-CAA-trnT-UGU_p5	trnL-CAA-trnT-UGU	5	CCCATTGTGAATTCCAGGGT	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	57.8	57.8	2.3	15.5	0.476982	14100	15740	1692-1817	1.000000	85.0
trnfM-CAU-trnG-GCC_p1	trnfM-CAU-trnG-GCC	1	GTAGCTCGCAAGGCTCATAA	TCTTTGCCAAGGAGAAGACG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.394901	25333	25482	237-257	1.000000	84.7
trnfM-CAU-trnG-GCC_p2	trnfM-CAU-trnG-GCC	2	TAGCTCGCAAGGCTCATAAC	TCTTTGCCAAGGAGAAGACG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.394901	25333	25482	236-256	1.000000	84.7
trnfM-CAU-trnG-GCC_p3	trnfM-CAU-trnG-GCC	3	TCTTCCGGAGTAGAGCAACT	TCTTTGCCAAGGAGAAGACG	20	20	50.0	50.0	57.8	57.8	3.3	0.0	0.387343	25333	25482	279-279	0.500000	56.9
trnfM-CAU-trnG-GCC_p4	trnfM-CAU-trnG-GCC	4	TCTTCCGGAGTAGAGCAACT	GCGGATGCGGATATAGTTGA	20	20	50.0	50.0	57.8	57.6	3.3	9.2	0.581298	25333	25482	312-312	0.500000	55.3
trnfM-CAU-trnG-GCC_p5	trnfM-CAU-trnG-GCC	5	TAGCTCGCAAGGCTCATAAC	GCGGATGCGGATATAGTTGA	20	20	50.0	50.0	57.8	57.6	0.0	9.2	0.588856	25333	25482	289-289	0.500000	55.3
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	CGTCTTCTCCTTGGCAAAGA	AGTGGGTATTCTTAATTCTCTCA	20	23	50.0	34.8	57.8	54.5	1.1	0.0	6.655566	25556	26238	761-896	1.000000	49.1
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	CGTCTTCTCCTTGGCAAAGA	AGTGGGTATTCTTAATTCTCTCATCT	20	26	50.0	34.6	57.8	57.4	1.1	0.0	6.729956	25556	26238	761-896	1.000000	49.1
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	CGTCTTCTCCTTGGCAAAGA	TGGGTATTCTTAATTCTCTCATCTCT	20	26	50.0	34.6	57.8	57.2	1.1	0.0	6.959599	25556	26238	759-894	1.000000	49.1
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	CGTCTTCTCCTTGGCAAAGA	AGTGGGTATTCTTAATTCTCTCATC	20	25	50.0	36.0	57.8	56.2	1.1	0.0	6.994171	25556	26238	761-896	1.000000	49.1
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	CGTCTTCTCCTTGGCAAAGA	GTGGGTATTCTTAATTCTCTCATCT	20	25	50.0	36.0	57.8	56.2	1.1	0.0	6.994171	25556	26238	760-895	1.000000	49.1
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.0	58.4	0.0	3.5	0.443887	26882	27090	408-411	1.000000	79.8
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GCTGAGTGGTTGATAGCTCC	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.1	58.4	0.0	3.5	0.475787	26882	27090	400-403	1.000000	79.6
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	GGCTGAGTGGTTGATAGCTC	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.1	58.4	0.0	3.5	0.475787	26882	27090	401-404	1.000000	79.6
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GGTTGATAGCTCCGGTCTTG	TAGGTCATTTATGGCACGCG	20	20	55.0	50.0	58.1	58.4	0.0	3.5	0.481207	26882	27090	393-396	1.000000	79.6
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	GCTTTTGGAGAGATGGCTGA	TAGGTCATTTATGGCACGCG	20	20	50.0	50.0	57.9	58.4	0.0	3.5	0.542333	26882	27090	415-418	1.000000	78.9
atpH-atpF_p1	atpH-atpF	1	ATTTGCGAATCCTTTTGTTT	GATGGCCAGTAACTCAAGCA	20	20	30.0	50.0	52.5	57.9	0.0	13.0	5.626499	49705	50306	671-741	1.000000	50.0
atpH-atpF_p2	atpH-atpF	2	ATTTGCGAATCCTTTTGTTT	TGGCCAGTAACTCAAGCAAA	20	20	30.0	45.0	52.5	57.6	0.0	0.0	5.869303	49705	50306	669-739	1.000000	50.0
atpH-atpF_p3	atpH-atpF	3	ATTTGCGAATCCTTTTGTTT	ATGGCCAGTAACTCAAGCAA	20	20	30.0	45.0	52.5	57.4	0.0	13.0	6.097474	49705	50306	670-740	1.000000	50.0
atpH-atpF_p4	atpH-atpF	4	ATTTGCGAATCCTTTTGTTT	CGGATGGCCAGTAACTCAAG	20	20	30.0	55.0	52.5	58.3	0.0	13.0	5.855986	49705	50306	673-743	1.000000	49.9
atpH-atpF_p5	atpH-atpF	5	ATTTGCGAATCCTTTTGTTT	ATCAAACCCGAAACTTCCGG	20	20	30.0	50.0	52.5	58.5	0.0	0.0	5.982315	49705	50306	693-763	1.000000	49.7
psbK-trnQ-UUG_p1	psbK-trnQ-UUG	1	AGCAGCTTGCCAAACAAAAG	TGCTTTCTTCGCCAGTCTTT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.088650	56450	57063	1531-1531	0.500000	59.3
psbK-trnQ-UUG_p2	psbK-trnQ-UUG	2	AGCAGCTTGCCAAACAAAAG	TTGCTTTCTTCGCCAGTCTT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.088650	56450	57063	1532-1532	0.500000	59.3
psbK-trnQ-UUG_p3	psbK-trnQ-UUG	3	CTTACAGCAGCTTGCCAAAC	TGCTTTCTTCGCCAGTCTTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.088776	56450	57063	1536-1536	0.500000	59.3
psbK-trnQ-UUG_p4	psbK-trnQ-UUG	4	CTTACAGCAGCTTGCCAAAC	TTGCTTTCTTCGCCAGTCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.088776	56450	57063	1537-1537	0.500000	59.3
psbK-trnQ-UUG_p5	psbK-trnQ-UUG	5	AGCAGCTTGCCAAACAAAAG	GAGTCTGAGTCTCAGTCGGA	20	20	45.0	55.0	58.1	57.9	0.0	25.7	0.158038	56450	57063	1468-1468	0.500000	58.7
accD_p1	accD	1	ATCGTCATTGATATGGGGCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.126145	57578	59377	2894-2894	0.500000	59.0
accD_p2	accD	2	ATAGTTGTGTCCCTAGCGGT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.165021	57578	59377	2418-2418	0.500000	58.7
accD_p3	accD	3	ATCGTCATTGATATGGGGCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	0.0	26.8	0.202678	57578	59377	2900-2900	0.500000	58.4
accD_p4	accD	4	ATAGTTGTGTCCCTAGCGGT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.1	57.9	0.0	26.8	0.241555	57578	59377	2424-2424	0.500000	58.1
accD_p5	accD	5	CATAGTTGTGTCCCTAGCGG	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	57.8	58.0	0.0	26.9	0.252072	57578	59377	2419-2419	0.500000	58.0
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTCTATTGT	AACTGGTATTCCCGTGATCG	20	20	45.0	50.0	56.5	57.4	0.0	0.0	2.111917	62861	64021	1298-1349	1.000000	75.1
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	AACTGGTATTCCCGTGATCG	21	20	42.9	50.0	57.2	57.4	5.1	0.0	2.359311	62861	64021	1220-1271	1.000000	73.1
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTCTATTGTT	AACTGGTATTCCCGTGATCG	21	20	42.9	50.0	57.2	57.4	0.0	0.0	2.361140	62861	64021	1298-1349	1.000000	73.1
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTCTATTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	56.5	57.1	0.0	4.1	2.402165	62861	64021	1248-1299	1.000000	72.8
petA-psbJ_p5	petA-psbJ	5	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGATCATCC	21	20	42.9	50.0	57.2	57.1	5.1	4.1	2.649558	62861	64021	1170-1221	1.000000	70.8
rps12-psbB_p1	rps12-psbB	1	CCCTCTTAGTCCGTTCTTGC	ACCAAAACAAGCTACCCCTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016164	70387	71669	2107-2766	1.000000	88.9
rps12-psbB_p2	rps12-psbB	2	CCCTCTTAGTCCGTTCTTGC	ATCAAAACCTTCCACACCCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021222	70387	71669	2224-2883	1.000000	88.8
rps12-psbB_p3	rps12-psbB	3	AGACAAAGAAATGGCGGGTT	ACCAAAACAAGCTACCCCTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.009247	70387	71669	2342-2342	0.500000	59.9
rps12-psbB_p4	rps12-psbB	4	AGACAAAGAAATGGCGGGTT	ATCAAAACCTTCCACACCCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.014305	70387	71669	2459-2459	0.500000	59.9
rps12-psbB_p5	rps12-psbB	5	AGACAAAGAAATGGCGGGTT	ACACCCATATTCCAGGACCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.024814	70387	71669	2391-2391	0.500000	59.8
rps8-rpl14_p1	rps8-rpl14	1	TCAGCATTGCGTATCAAGGT	AGTTTCATTAGCACCTGAGGT	20	21	45.0	42.9	57.6	56.9	2.4	0.0	2.479639	80043	80824	775-860	1.000000	71.8
rps8-rpl14_p2	rps8-rpl14	2	GTCAGCATTGCGTATCAAGG	AGTTTCATTAGCACCTGAGGT	20	21	50.0	42.9	57.3	56.9	2.4	0.0	2.822843	80043	80824	776-861	1.000000	69.0
rps8-rpl14_p3	rps8-rpl14	3	TCAGCATTGCGTATCAAGGT	GTTTCATTAGCACCTGAGGT	20	20	45.0	45.0	57.6	55.4	2.4	0.0	3.049255	80043	80824	774-859	1.000000	67.2
rps8-rpl14_p4	rps8-rpl14	4	TCATGTCAGCATTGCGTATCA	AGTTTCATTAGCACCTGAGGT	21	21	42.9	42.9	58.1	56.9	2.4	0.0	3.174258	80043	80824	780-865	1.000000	66.2
rps8-rpl14_p5	rps8-rpl14	5	CAGCATTGCGTATCAAGGTT	AGTTTCATTAGCACCTGAGGT	20	21	45.0	42.9	56.8	56.9	2.4	0.0	3.289470	80043	80824	774-859	1.000000	65.3
rpl23-ycf2_p1	rpl23-ycf2	1	ATAGGTCGCCCTCTTCTACC	GCTAGGTCCAATGGAATGCA	20	20	55.0	50.0	58.0	57.9	0.0	2.3	0.058915	86228	87306	1621-1621	0.500000	59.5
rpl23-ycf2_p2	rpl23-ycf2	2	ATAGGTCGCCCTCTTCTACC	CTAGTGGACCCCTCAGTAGG	20	20	55.0	60.0	58.0	57.9	0.0	0.0	0.076506	86228	87306	1851-1851	0.500000	59.4
rpl23-ycf2_p3	rpl23-ycf2	3	ATAGGTCGCCCTCTTCTACC	CAAAATTCGGGGGTCCAAGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.083906	86228	87306	1447-1447	0.500000	59.3
rpl23-ycf2_p4	rpl23-ycf2	4	ATAGGTCGCCCTCTTCTACC	GAGTCCTCTGTGAATTGCGT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.087832	86228	87306	1481-1481	0.500000	59.3
rpl23-ycf2_p5	rpl23-ycf2	5	ATAGGTCGCCCTCTTCTACC	GGGGGTCCAAGAGTTTGATC	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.132050	86228	87306	1439-1439	0.500000	58.9
trnL-UAA-trnN-GUU_p1	trnL-UAA-trnN-GUU	1	GTCTCGCGTGTCTACCATTT	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	58.0	58.2	5.8	15.0	0.164462	93809	94488	774-919	1.000000	85.5
trnL-UAA-trnN-GUU_p2	trnL-UAA-trnN-GUU	2	CTCGCGTGTCTACCATTTCA	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	58.0	58.2	5.8	15.0	0.165833	93809	94488	772-917	1.000000	85.5
trnL-UAA-trnN-GUU_p3	trnL-UAA-trnN-GUU	3	TACCATTTCACCACCAAGGC	AGGATTCGAACCTACGACCA	20	20	50.0	50.0	58.1	58.2	0.0	15.0	0.238760	93809	94488	762-907	1.000000	85.0
trnL-UAA-trnN-GUU_p4	trnL-UAA-trnN-GUU	4	GTCTCGCGTGTCTACCATTT	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	58.0	57.7	5.8	0.0	0.335030	93809	94488	782-927	1.000000	84.1
trnL-UAA-trnN-GUU_p5	trnL-UAA-trnN-GUU	5	CTCGCGTGTCTACCATTTCA	CCCCAAGTAGGATTCGAACC	20	20	50.0	55.0	58.0	57.7	5.8	0.0	0.336401	93809	94488	780-925	1.000000	84.1
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	94562	95171	689-703	1.000000	83.7
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.483095	94562	95171	693-707	1.000000	82.8
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	57.7	57.8	0.0	18.9	0.543107	94562	95171	681-695	1.000000	82.4
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.526686	94562	95171	732-746	1.000000	82.1
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.3	57.8	4.4	18.9	0.876096	94562	95171	690-704	1.000000	79.6
ndhB-ycf1_p1	ndhB-ycf1	1	ATGCTTGCGTATTCGTCCAT	CCCTTCTTCCATAAACCGGG	20	20	45.0	55.0	58.0	57.9	0.0	6.3	0.099223	108289	109036	1181-1483	1.000000	88.5
ndhB-ycf1_p2	ndhB-ycf1	2	GGACTGGCATCTTCTTCTGG	CCCTTCTTCCATAAACCGGG	20	20	55.0	55.0	58.0	57.9	0.0	6.3	0.088312	108289	109036	1269-1571	1.000000	88.4
ndhB-ycf1_p3	ndhB-ycf1	3	GGACTGGCATCTTCTTCTGG	CCCCCTTCTTCCATAAACCG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.088312	108289	109036	1573-1573	0.500000	59.3
ndhB-ycf1_p4	ndhB-ycf1	4	GGACTGGCATCTTCTTCTGG	CCCCTTCTTCCATAAACCGG	20	20	55.0	55.0	58.0	57.9	0.0	0.0	0.088312	108289	109036	1572-1572	0.500000	59.3
ndhB-ycf1_p5	ndhB-ycf1	5	ATGCTTGCGTATTCGTCCAT	CCCCTTCTTCCATAAACCGG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.099223	108289	109036	1484-1484	0.500000	59.2