Hotspot

ndhF

SSC · 108846-111092 · 2247 bp

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Pi 0.0105

MarkerSeek score 48.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 71
pi: 0.0105
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAATCGTCCAACGCGATAT	AGAGACAAGAGCCAGTGAATG	50.0	47.6	58.2	57.7	2671 bp	1.0	69.8
ndhF_p2	GGAAGAAGAGGGATTCACCG	AGAGACAAGAGCCAGTGAATG	55.0	47.6	57.7	57.7	2961 bp	1.0	69.0
ndhF_p3	ACTTCCGAAACGAAGGAGAC	AGAGACAAGAGCCAGTGAATG	50.0	47.6	57.6	57.7	2987 bp	1.0	68.0
ndhF_p4	CCGAGTGAATGGAAAGGACA	AGAGACAAGAGCCAGTGAATG	50.0	47.6	57.5	57.7	2862 bp	1.0	67.8
ndhF_p5	GGAATCGTCCAACGCGATAT	AAGAGACAAGAGCCAGTGAA	50.0	45.0	58.2	56.4	2672 bp	1.0	67.7