Hotspot

rrn23-rrn4.5

IRb · 105501-105592 · 92 bp

Back to Chenopodium

Pi 0.0401

MarkerSeek score 54.00

Primer available yes

Label rrn23-rrn4.5

Feature Metadata

label_name: rrn23-rrn4.5
feature_type: igs
region: IRb
length_bp: 92
variable_sites: 7
pi: 0.0401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn4.5_p1	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	50.0	50.0	58.2	58.0	186 bp	1.0	80.6
rrn23-rrn4.5_p2	AAGTAGTAAGCCCACCCCAA	CTGTGGGTCTGTTAGGATGC	50.0	55.0	58.2	58.0	220 bp	1.0	80.3
rrn23-rrn4.5_p3	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	50.0	55.0	58.2	58.0	212 bp	1.0	80.2
rrn23-rrn4.5_p4	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	50.0	55.0	58.2	58.1	214 bp	1.0	80.2
rrn23-rrn4.5_p5	TAGTAAGCCCACCCCAAGAT	CTGTGGGTCTGTTAGGATGC	50.0	55.0	57.7	58.0	217 bp	1.0	80.1